Laboratory Procedure of CSF Glucose test.

The laboratory procedure for a CSF glucose test involves collecting a sample of cerebrospinal fluid (CSF) and measuring the concentration of glucose in the sample. Here is a general overview of the laboratory procedure for CSF glucose testing:

1. Collection of CSF Sample: A lumbar puncture (spinal tap) is commonly performed to collect CSF.

Centrifugation: If the CSF sample contains blood cells, it is centrifuged to separate the cells from the CSF. The supernatant, which contains the CSF, is then used for further analysis.

2. CSF Glucose Analysis: The collected CSF sample is transferred to a sterile tube.

The glucose analysis uses specific chemical assays or enzymatic methods.

3. Glucose Measurement: Different methods can be used to measure glucose levels in CSF, including colorimetric methods or enzymatic assays.

In colorimetric methods, the reaction between glucose and specific reagents produces a color change, which is then measured by a Colorimeter.

Enzymatic assays involve the use of enzymes that react specifically with glucose, producing a measurable change.

4. Reference Range: The normal range for CSF glucose is 45-80 mg/dL. Lower CSF glucose levels may indicate meningitis, encephalitis, or subarachnoid hemorrhage. Elevated CSF glucose levels may be seen in patients with hyperglycemia or certain types of tumors.

5. Interpretation: The interpretation of the CSF glucose test results should be considered in conjunction with other clinical findings and laboratory tests. An abnormal CSF glucose level may warrant further investigation to determine the underlying cause.