Clinical Chemistry (General) Suggestion - january 2018

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1.     Define ELISA. Write the principle and component of ELISA. What are the tests done by ELISA?

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1.     Define OD. How can you measure OD of any solution by a colorimeter or spectrophotometer?

2.     What is Haemoglobinure?  State the causes of Haemoglobinur.

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1.     Define normal range or reference rang.  Write down the reference range of BS, s creatinine, s calcium, Blood urea, S cholesterol, Triglycerides, UTV, UTP, relative mass density, glucose, bilirubin, HDL, LDL, BUN, alkaline phosphate, Uric acid.

2.     What is solution? How can you prepare 200ml of 5% Nacl solution?

3.     Give the definition and causes of hyperglycemia and hypoglycemia.

4.     Define quality control. How can you maintain quality control in a clinical chemistry laboratory?

5.     Write down the biological factors affecting the test result.

6.     Give the difference between spectrophotometer and colorimeter.

7.     Write down the principle and procedure of serum creatining estimation.

8.     What is reagent strips? How can you maintain quality control of reagent strips?

9.     Define acid, base and alkali. Explain that all alkalis are bases but all bases are not alkalis.

10.  What is chemical reaction? State the causes of chemical reaction.

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1.     How can you detect blood sugar in your lab?

2.     Write down the procedure of urea estimination.

3.     What is electrolyte? List the common electrolytes with their normal range.

4.     What is Bence Jonce protein? How can you detect it urine.

5.     Draw and label an autopipette. Mention the uses of autopipette.

6.     What is salt and acid? Classify acid with examples.

7.     Define lipid profile. Write down the biochemical importance of lipid profile.

8.     What is diabetes? Write down the investigations in diabetes mellitus.

9.     How will you store and safely use of chemical and reagents in clinical chemistry laboratory.

10. What are the causes of massive protein urea?

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1.     What is lipid profile? Describe the procedure of serum cholesterol estimation.

2.     How can you detect albumin in urine?

3.     Define buffer, normal and standard solution.

4.     What do you mean by Uremia? Write the causes of urea.

5.     How can you prepare buffer solution in laboratory?

6.     What is urinometer? How can you measure relative mass density of urine sample?

7.     What is plasma? How can you obtain serum and plasma from blood?

8.     Define S.I. unit. State the base SI unit with their symbol and measurement.

9.     Define laboratory safety. State the laboratory hazard.

10.  What is ICT? How can you perform HBSAG test by ICT method.

11.   Name the five instruments and reagent used in clinical chemistry laboratory.

12.  How can you measure P^H of a solution?

13.  How can you prepare 300ml of 5% Hcl solution?

14. What is haemolysis? State the causes of haemolysi.

15.  Define ketonuria. Mention the causes of ketonuria in urine.

16.  How can you detect ketonuria in urine?

17.  What is uremia? What are the causes of abnormal of label of urea in urine?

18. What is dilution? How will you dilute a solution?

19. Define protineurea. Massive proteinurea and microalbuminuria.

20. State the methods of chemical reaction.

21.Calculate the molecular weight an equivalent weight of H₂SO₄ and HNO_.

22.Define hypotonic & hypertonic solution.

23.What is hypoglycaemia? Write down the causes of hypoglycaemia.

24.Define buffer solution. How can you prepare of 500ml 5% Hcl solution?

25.Write down the principle and procedure of serum bilirubin test.

26.Name ten instruments use in laboratory.

27.What do you mean by ELISA?

28.What is haemoglobinurea? What are the causes of haemoglobinure?

29. How can you establish reference range in the laboratory?

30.What is importance of blood bufferaction?

31.Name the blood buffer.

32.Draw the different parts of colorimeter. Write its clinical importance.

33.Define molor and normal solution

34.Write down the application of SI unit in the laboratory.

35. Write down the procedure of testing urine for hb.

36.What is haemoglobinurea? What are the causes of haemoglobinure?

37.What is diabetes mellitus? Write down the investigations in diabetes mellitus.

38.Write down the increase and decreased level of cholesterol.

39.Make 500ml of NaOH, 0.25 mol/liter from a 0. 4 mol/liter solution.

Short note:-

    1.Indicator.

2.OGTT.

           3.Autoanalizer.

                     4.Spectrophotometer.

                                        5.Quantity controlling laboratory.

                    6.Flame photometer.

                     7.Renal function test.

          8.Urinometer.

                  9.Percent solution.